Dr. Carien Coetzee
Basic Wine
24 November 2020


Ladybug infestations in 2001 led to major product loss in grape-growing areas such as Ontario. Linda Franklin, executive director of the Wine Council of Ontario, a trade association of wineries, said that about 20% of the province’s wine production was affected. The Ontario wine industry had to discard more than a million litres of wine as they did not want to the release tainted wine, thereby protecting the reputation of their industry.

The effects of ladybug taint can be potentially detrimental and although it is not a problem in the South African wine industry, producers need to familiarise themselves with ladybug taint to be prepared to prevent and treat the threat when needed.  Part 2 of this blog series will briefly look at options to avoid/remediate the taint.


How to prevent ladybug taint


The ladybug-derived methoxypyrazines are extracted from the bugs relatively quickly (within 24 hours of maceration) and it is important to 

  1. limit the number of ladybugs present on the grapes before processing
  2. minimise the duration of contact during processing

Ladybug populations can be controlled by limiting their primary food source, mainly aphids. Large infestations will not occur if the food source is scarce. The use of pesticides can be used to control food sources such as aphids as well as beetle populations. Good weed and cover crop management within the vineyard can also help control insect populations.

The harlequin beetle does not cause initial berry injury1, however, berry damage due to other factors can potentially support a growing population. Therefore, growers should closely monitor the incidence of berry injury and addition to infestations in clusters.

If present on the grapes during harvest, processes such as destemming will help to remove some of the insects from the fruit. Minimising skin contact and pressing as quickly as possible will reduce the available time for the secretion and extraction of methoxypyrazines from the ladybugs present.


Reducing the taint


Other treatments that might help reduce the unwanted methoxypyrazines have been tested on juice and wine with generally limited or mixed results. Some of these include:


Clarifying the juice before alcoholic fermentation


Settling and clarification of the juice before fermentation is an effective tool to decrease the methoxypyrazine concentration2. Thorough settling and racking of the juice before fermentation can reduce methoxypyrazine content by more than half, however, it should be noted that in this particular study, the initial turbidity of the juice (before settling) was 1280 ntu2.




Thermo-vinification has been shown to be effective at reducing methoxypyrazine content in must3. Heating the must for a short period between 60°C – 80°C can potentially reduce the concentration by up to 67%4 (most likely by evaporation loss). However, this is not a practice typically done on Sauvignon blanc must due to unfavourable effects impacting the wine quality.


Fining with silicone and polylactic acid5


Even though it is not traditionally used in winemaking processes, the addition of silicone and a polylactic acid-based polymer resulted in a significant decrease in methoxypyrazines without altering the desirable aromas5–8. The exact protocol for the use of these polymers still needs to be developed, however, there are several potential applications and uses. Polylactic acid can be manufactured in a variety of forms with different physical properties. Due to this flexibility of processing, the product can be integrated into existing filtration systems, manufactured as inserts for larger tanks or added as pellets directly to the juice and/or wine6,9.


Fining with mousemajor urinary protein


An odorant-binding protein (mMUP) with high specificity for methoxypyrazines can reduce levels from 300 ng/L to just 5 ng/L7,10. The protein binds the methoxypyrazines and is then removed from the must or wine by fining with bentonite. Product development and optimization are underway at Brock University, Ontario, Canada9.


Light treatment and irradiation


Some studies have found exposure to sunlight to reduce the methoxypyrazine concentration in wine, however other studies reported no consistent effects from light exposure or bottle colour11–13. The indirect effect of sunlight exposure also needs to be considered as sunlight treatment can lead to increases in temperature which can have detrimental effects on wine quality.


Reduce the perception of the methoxypyrazines (sensory masking)


Certain winemaking practices such as fermenting with specifically selected yeast strains and/or the use of oak could lead to the formation and/or extraction of other flavour compounds that could help mask some of the aromas contributed by methoxypyrazines. Yeasts differ in their ability to produce aroma compounds and by selecting a yeast that will contribute fruity aromatics (for example), the inherent greenness of the wine can be masked. Thus, the methoxypyrazine concentration will largely remain unchanged, however, the sensory perception of the compounds will be suppressed. The effectivity of these approaches will depend on the type and concentration of the masking compounds as well as the concentration of methoxypyrazines present.

Blending a ladybug tainted wine with a wine containing no methoxypyrazines and/or high concentrations of masking compounds is probably one of the most useful tools to dilute and mask the taint.  Sensory interactive effects still need to be considered and blending trials are advised.




While no infestations have been publicly reported in South African vineyards, knowing the potential harm of lady beetles is important for grape growers and winemakers to be best prepared and able to respond if an infestation were to occur.

Remedial treatments show low success rates, therefore ensuring the viticultural and winemaking team is informed is key to spot and prevent the problem from manifesting.




(1)           Koch, R. L.; Burkness, E. C.; Burkness, S. J. W.; Hutchison, W. D. Phytophagous Preferences of the Multicolored Asian Lady Beetle (Coleoptera: Coccinellidae) for Autumn-Ripening Fruit. Journal of Economic Entomology 2004, 97 (2), 539–544. https://doi.org/10.1603/0022-0493-97.2.539.

(2)           Kotserides, Y.; Spink, M.; Brindle, I. D.; Blake, A.; Sears, M.; Chen, X.; Soleas, G.; Inglis, D.; Pickering, G. J. Quantitative Analyses of 3-Alkyl-2-Methoxypyrazines in Juice and Wine Using Stable Isotope Labelled Internal Standard Assay. Journal of Chromatography A 2008, 1190, 294–301.

(3)           Kögel, S.; Botezatu, A.; Hoffmann, C.; Pickering, G. Methoxypyrazine Composition of Coccinellidae-Tainted Riesling and Pinot Noir Wine from Germany. Journal of the Science of Food and Agriculture 2014, 95 (3), 509–514. https://doi.org/10.1002/jsfa.6760.

(4)           Kögel, S.; Botezatu, A.; Hoffmann, C.; Pickering, G. Methoxypyrazine Composition of Coccinellidae-Tainted Riesling and Pinot Noir Wine from Germany. Journal of the Science of Food and Agriculture 2015, 95 (3), 509–514. https://doi.org/10.1002/jsfa.6760.

(5)           Botezatu, A.; Pickering, G. J. Application of Plastic Polymers in Remediating Wine with Elevated Alkyl-Methoxypyrazine Levels. Food Additives and Contaminants – Part A Chemistry, Analysis, Control, Exposure and Risk Assessment 2015, 32 (7), 1199–1206. https://doi.org/10.1080/19440049.2015.1028106.

(6)           Ryona, I.; Reinhardt, J.; Sacks, G. L. Treatment of Grape Juice or Must with Silicone Reduces 3-Alkyl-2-Methoxypyrazine Concentrations in Resulting Wines without Altering Fermentation Volatiles. Food Research International 2012, 47 (1), 70–79. https://doi.org/10.1016/j.foodres.2012.01.012.

(7)           Botezatu, A. I.; Kotseridis, Y.; Inglis, D.; Pickering, G. J. Occurrence and Contribution of Alkyl Methoxypyrazines in Wine Tainted by Harmonia Axyridis and Coccinella Septempunctata. Journal of the Science of Food and Agriculture 2013, 93 (4), 803–810. https://doi.org/10.1002/jsfa.5800.

(8)           Botezatu, A.; Kemp, B. S.; Pickering, G. J. Chemical and Sensory Evaluation of Silicone and Polylactic Acid-Based Remedial Treatments for Elevated Methoxypyrazine Levels in Wine. Molecules 2016, 21 (9). https://doi.org/10.3390/molecules21091238.

(9)           Pickering, G. J. Managing Green Flavors in the Winery. Wines & Vines Analytics 2018.

(10)        Inglis, D.; Beh, A. L.; Brindle, I. D.; Pickering, G.; Humes, E. F. Method for Reducing Methoxypyrazines in Grapes and Grape Products. 13/264426, 2014.

(11)        Blake, A.; Kotseridis, Y.; Brindle, I. D.; Inglis, D.; Pickering, G. J. Effect of Light and Temperature on 3-Alkyl-2-Methoxypyrazine Concentration and Other Impact Odourants of Riesling and Cabernet Franc Wine during Bottle Ageing. Food Chemistry 2010, 119 (3), 935–944. https://doi.org/10.1016/j.foodchem.2009.07.052.

(12)        Maga, J. A. Sensory and Stability Properties of Added Methoxypyrazines to Model and Authentic Wines. In Flavors and Off Flavors: Proceedings of the 6th International Flavor Conference; Amsterdam, 1990; pp 61–70.

(13)        Pickering, G. J.; Lin, J.; Reynolds, A.; Soleas, G.; Riesen, R. The Evaluation of Remedial Treatments for Wine Affected by Harmonia Axyridis. International Journal of Food Science and Technology 2006, 41, 77–86.

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